FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020663
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13950.039.7627941
TCGCTCT13900.038.332014
TCTGTCG14800.032.595518
CTCGCTC17400.030.621553
CGCTCTG16450.029.326055
GTCTTGC32600.028.94771
TCTCGCT18900.028.5722272
GTACTAG5850.027.0911371
GTGTTAG20800.027.0141741
GTGTAAA20600.025.8776531
CTGTCGC20250.025.2451539
TCGACCA2351.414317E-624.5113269
GTATTAT10150.024.1308861
GTGTAAC9950.023.8919321
GTTTCAC51800.023.0740053
GGTATAC5950.023.0036661
GTCTTAC13550.022.8607031
GGGTTTC58350.022.8397461
TTCACCG10150.022.7001465
TTGCTCT41050.022.2758984
Produced by FastQC (version 0.11.5)