FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373650
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.046.0195478
CTGTCGC12600.039.4276549
GTCTTGC22400.037.6086431
GTCTCGC10550.037.5370451
TCCGTCG1102.53854E-432.7265138
TCGCTCT11150.029.7033464
TCTCGCT12600.028.001122
TTGCTCT28850.027.9508234
CGTTTCG1607.162694E-526.9993729
GTGTAGG12400.025.5494061
GTATTAC4800.025.5010191
ACCGTGT7700.025.2461668
CTTGCTC30400.025.105743
TACACCG2901.2838427E-824.827015
CTCGATC8750.024.685148
TACCCGT1751.3170826E-424.6851395
CTCGCTC13500.024.5337963
GTCTCAC28650.024.3779351
TCGATCT8900.024.2690989
GTGTAGA11150.023.8933321
Produced by FastQC (version 0.11.5)