FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297663
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10000.040.3187378
GTCTCGC10700.034.319671
CTGTCGC13000.033.7835589
TCGCTCT10700.032.971654
GTCTTGC21250.031.8511871
CTCGCTC13750.026.7069443
GTATATA9650.026.1154461
TCTCGCT13900.025.9007242
GTGTAAA12600.025.715721
GTATAAT6500.025.4783441
GTACTCG2301.1722277E-625.0448761
GTTCGGA1751.316358E-424.687091
GTATAAG5900.024.408141
GTATAGA7800.024.0013391
GCGTTAT1500.001555824324.0013371
GGTATAC4002.910383E-1123.4013061
GTATAGC5250.023.3155861
GTATAGG4650.023.2271021
GTGTATA10650.022.9871981
GTGTTAC10200.022.5894971
Produced by FastQC (version 0.11.5)