FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297663
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10900.031.705878
GTCTTGC21600.031.015661
GTCTCGC9800.030.1376671
GTATTAG7350.029.4026011
TCGCTCT10800.029.3328234
TATACCG1000.00628553428.7994985
GTTCGGA1555.7493642E-527.8850461
GTATAGG5100.026.837081
GTATAGA6300.026.2989921
GTGTATA10350.025.0561281
CTGTCGC13800.025.0430439
TCTCGCT12750.024.8471662
GTGTTAC8500.023.7296281
CTCGCTC13300.023.2782963
GTATATA8750.023.0516381
GTGTTAG15050.022.9750561
TACACTG12750.022.5878435
CTTGCTC30350.022.5371653
CGCTCTG11850.022.4806215
GTCTTAC9300.022.4629551
Produced by FastQC (version 0.11.5)