FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3913423
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16050.040.3733568
GTCTCGC18600.038.711691
TCGCTCT21600.035.3333664
GTCTTGC39100.033.6999661
CTGTCGC20500.032.3118139
TCTCGCT23450.030.398172
CGCTCTG22950.029.4902255
CTCGCTC25600.028.1264653
TTGCTCT48700.025.281344
GTACTAC7200.025.0013031
GTCTCAC47000.024.3587151
GTATTAA11750.023.8991171
GGGTTTC67200.023.0369131
GTGTTAG26550.022.7808481
CTTGCTC49000.022.7766953
GTGTAAC10950.022.3573281
GAGTGCA100150.022.2146996
GGAGTGC94200.021.783465
GTATTAG11600.021.7252711
TCTGTCA50600.021.4859128
Produced by FastQC (version 0.11.5)