FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464881
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12600.035.4266748
GTCTCGC12400.033.0978661
CGGTACT1753.9880615E-628.7992884
CTGTCGC16450.027.572999
TCGCTCT15350.027.204544
GTCTTGC31300.025.9944691
GTGTAAG17050.025.7603821
GTGTATA19200.025.125831
GTATATA15350.023.9226131
GTCTTAC13500.022.934091
GGTATAC6950.022.7921181
CGCTCTG18400.022.6950915
GTGTTAG23450.022.4143851
GTATAAC6800.022.2360271
CTCGCTC18800.022.2131793
GTGTTAT13050.022.0696931
GTCTCAC42300.021.958171
GTATAAT11850.021.874141
TCTCGCT17500.021.8064332
GTACTAG6950.020.7201081
Produced by FastQC (version 0.11.5)