FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136645
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14050.047.1452458
CTGTCGC18800.037.5313879
GTCTCGC15350.035.181461
TCGCTCT16750.030.9498024
GTATAGC6050.030.9441811
GTCTTGC30050.030.4312841
TCTCGCT17500.029.2133452
GTATTAC6850.028.3814261
CGCTCTG18400.028.1744165
CTCGCTC17400.028.139333
GTGTTAC12800.027.564311
TTGCTCT40650.026.568744
GGTATAC6900.025.0451241
TAATACT10450.024.1152634
GTATTAG9600.024.0015741
CTTGCTC38100.024.0011923
GTGTAGG18000.022.4014721
GTCTCAC35250.022.2652931
GTATTAA9750.022.1553021
GTACTAG5250.021.94431
Produced by FastQC (version 0.11.5)