FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136645
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14750.040.5150878
GTCTCGC12250.037.039641
TCGCTCT14150.032.56424
GTCTTGC28850.032.2037281
CGCTCTG15300.029.1754235
CTGTCGC20500.029.1511029
TAGTCCG1504.615492E-528.7989655
TCTCGCT16050.026.9153482
CTCGCTC16400.025.901923
GTGTTAG21150.024.5179631
GTTTCAC51950.024.3921953
GTATTAT10200.024.0071721
GGGTTTC57400.023.5889281
GGGTAAG14750.023.437511
GTACGGC1550.001880396923.2327481
GTACAAG11300.022.9449081
GAGTGCA78050.022.876826
GTCCTAC9600.022.5067231
GTATTAC6750.022.4066961
GTATAAT9000.022.4066961
Produced by FastQC (version 0.11.5)