FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911913
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11800.034.7795758
GTCTCGC14400.031.999921
GTCTTGC29350.031.4002651
CTGTCGC14400.029.9999269
GTATTAG8700.028.9654451
TCGCTCT13750.027.7526574
GTATAGA8550.026.94731
GTTTCAC47150.026.417753
GTATTAC6700.025.7909811
GTCTCAC34450.025.7067571
GTGTTAA12350.025.6517581
GGGTTTC51750.025.5999361
TTGCTCT38600.025.554344
TTCACCG9750.024.369175
GTATATA11000.024.218121
GTACGGT2401.6991762E-623.999946
GTCTTAC10350.023.6521151
GTGTATA13450.023.5538461
TACACTG18150.023.40495
GTATAGT8950.023.329551
Produced by FastQC (version 0.11.5)