FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911913
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13150.042.180631
TCTGTCG14000.037.0278748
TCGCTCT16550.032.1927454
CTGTCGC18650.031.656249
GTCTTGC26950.031.2734071
CGCTCTG17250.029.216845
CTCGCTC16900.028.5477623
TCTCGCT17300.027.8944052
GTCTCAC35400.025.8433341
GTGTTAT10150.025.5496231
GTATTAG8750.025.5212361
GTCTTAC11450.025.1653291
GTATAAG7300.024.6697771
GTGTTAC12600.024.0119151
GTGTAAC8200.023.719091
GTGTTAG18350.023.5539251
GTACGTA2202.3442719E-522.9204671
GGTATAC6000.022.8113211
GTGTAAA16600.022.5654181
GTACAAG10550.022.532511
Produced by FastQC (version 0.11.5)