FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863949
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10800.041.332538
GTCTTGC24150.030.4258161
GTACTAA5900.029.3034251
CTGTCGC16000.028.7994389
GTCTCGC10050.027.2380961
TCGCTCT11750.025.122924
GGAGTGC60650.024.4545975
GTATTAA7100.024.3507351
GTATTAT9200.024.2735371
GTGTAAC7500.024.0125311
GAGTGCA64500.023.6646566
GTCCTAC7350.023.5224781
GTGTTAT10150.023.421091
GTCTTAC9850.023.4030761
GTCTCAC28500.022.7487141
TTATACT9500.022.73644
GTGTTAG15400.022.4532761
GTTACCC9000.022.4030863
TTGCTCT32100.022.2051774
GTATTAG7800.022.1654111
Produced by FastQC (version 0.11.5)