FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3282010
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15250.035.4296651
TCTGTCG14850.030.5448658
TCGCTCT16250.028.3563754
GTCTTGC29300.027.5375981
CTCGCTC19150.024.4422683
GTGTACG3001.8297214E-824.013441
TCTCGCT19500.024.0072172
CTGTCGC19800.023.9995389
CGCTCTG16900.023.8575295
GTGTTAC13000.023.8287221
GTCTTAC13250.023.3791221
GTATTAA8650.023.319411
GTGTTAG19600.022.7882631
GGGTACG2954.1250496E-721.9784031
GTGTATA14650.021.1449411
GTATACG2053.829922E-421.0849721
GTTTCAC49800.020.9669653
GTATAAG10450.020.6814331
TTCACCG9750.020.6765255
GTCTCAC34750.020.3164061
Produced by FastQC (version 0.11.5)