FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147075
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12800.038.249978
GTCTCGC14450.037.8684841
TCGCTCT15200.031.7368164
TCTCGCT17150.030.2273852
GTCTTGC29800.029.7180981
CTGTCGC17150.029.3877359
CTCGCTC17450.028.8824983
GTATAAC5500.027.4908871
GTATAGA9550.026.3874151
GTCTTAC11600.025.4482561
GTATTAC7600.024.631561
GTATTAG9950.024.6029971
GTGTTAG20650.024.4067611
CGCTCTG19400.024.1236925
TTGCTCT39750.022.8226244
TACACTG17150.022.6705385
GGTATAC7100.022.3098431
GTATAGG8750.022.2171251
GTCCTAC9750.022.153831
GTATAAG9450.022.0952221
Produced by FastQC (version 0.11.5)