FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147075
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14200.038.0272678
GTCTCGC15700.035.7903251
TATACCG1301.7364919E-533.2299845
TCGCTCT16500.033.162854
GTCTTGC28950.031.3539751
CTGTCGC19700.029.603369
GGTATAC5750.028.8157981
TCTCGCT18900.027.8181482
GTATTAG8600.027.6430631
CTCGCTC18750.026.8849143
CGCTCTG20200.026.7320385
GTATAAT7850.026.613321
GTATAAG8350.025.0197031
GTGTTAG22800.023.6972031
TATACGG1550.001883930123.2252565
GTCTTAC11600.022.9781151
GAGTGCA83000.022.5536826
GGAGTGC78700.022.3222165
GGGTAAT10400.022.1659981
GTATTAC8250.021.830151
Produced by FastQC (version 0.11.5)