FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3502779
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15600.033.2453771
TCTGTCG13250.030.9730458
TCGCTCT17850.028.6381574
GTCTTGC32000.028.1373621
GTATTAC9200.025.837441
CTCGCTC19600.025.7182433
CGCTCTG19800.025.4541025
TCTCGCT20300.025.1900962
CTGTCGC18050.023.93319
GTGTTAC13100.023.6439741
GGTATAC7650.023.5397551
GTGTTAG22550.023.3184491
GTATATA13950.023.2360151
GTATTAG11250.023.0501251
GGAGTGC76050.022.4374795
GAGTGCA81300.022.4055146
GTATAGT9500.021.9886071
CTATACG1650.002709087521.81784
GTGTAAT13750.021.4785271
GTACGGG2704.707166E-621.342711
Produced by FastQC (version 0.11.5)