FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597130
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.048.8240368
GTCTCGC12900.040.7441861
TCGCTCT13200.038.7272724
CTGTCGC17200.035.1627929
GTCTTGC25550.033.2524451
TCTCGCT14550.033.154642
CGCTCTG15150.032.316835
GTCTAAC5700.031.5789491
CTCGCTC16000.029.2499983
GTATTAA7450.027.0604041
GTACCGT1907.5566477E-626.5263161
GTGTTAC9100.026.1098921
GGTATAC4750.025.7684211
TAGTACT5250.024.6857154
GTGTTAG17300.024.138731
TACACTG15400.023.3766235
TCTGTCA33500.023.211948
GTATTAG6950.022.7913681
GTATAAT5700.022.7368431
GTATAGG5750.022.5391311
Produced by FastQC (version 0.11.5)