FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597130
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13450.035.879521
TCTGTCG12650.035.2880828
TCGCTCT11650.033.990984
GTCTTGC26250.031.2802091
CTGTCGC17100.028.2101579
GTATTAA7150.028.2062991
CTCGCTC14550.027.219813
TCTCGCT15100.027.1847362
GTATTAC5950.026.6317581
TTGCTCT34050.025.5855714
GTGTTAA9600.025.5094911
GGTATAC4101.8189894E-1224.5945191
GAGTGCA67650.024.1593326
GGAGTGC64300.023.5144715
CGCTCTG15650.023.4629555
GTACCGT1851.9198841E-423.3510516
GTGTACG3402.7066562E-923.302791
GTATAGT5900.023.1950721
GTGTTAG17400.022.7670921
GTCTCAC30100.022.7327121
Produced by FastQC (version 0.11.5)