FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3101853
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11150.036.806998
TCGCTCT13300.035.1877984
GTCTTGC21500.034.493971
CTGTCGC13450.032.11889
GTCTCGC12850.031.3782861
CGCTCTG14550.028.2060475
TCTCGCT15400.028.0518112
GTCTCAC30050.028.0340421
CTCGCTC15250.027.383473
GTCTAGC11100.027.2439881
GTATAGG6200.025.5490861
TCTGTCA31550.025.1028868
TTGCTCT32500.024.5906474
GGAGTGC62600.024.4982815
GGCTGGA88300.023.2395941
GTGTTAC10350.022.9571481
CTGTCAC34850.022.9324539
CTGGAGT77300.022.8200683
GAGTGCA67900.022.4800076
TCACTCT33900.021.8759984
Produced by FastQC (version 0.11.5)