FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145088
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14350.039.1350258
GTCTCGC14450.036.885821
GTCTTGC29200.035.766851
TCGCTCT16250.032.786974
CTGTCGC18800.028.3397989
CGCTCTG19100.027.894675
GTATAAT9150.027.5513251
CTCGCTC18950.027.3616873
TTGCTCT41850.027.3542354
GTATTAA10100.027.0992831
TCTCGCT20000.027.0079922
GTGTTAC11750.025.7458361
GTGTTAG20550.025.5862461
ACCGTGT11600.025.4477148
GTGTAAC9400.025.2860871
GTGTAAT12250.025.282961
TTCACCG10250.023.88245
GTATAGG8750.023.8718191
GTCTTAC13000.023.8243271
CTTGCTC40600.023.7683123
Produced by FastQC (version 0.11.5)