FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737812
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13250.035.1582681
TCGCTCT13400.034.9222874
TCTGTCG12300.033.9488378
GTCCGAT1000.005959461429.115441
GTCTTGC25100.028.9994431
CGCTCTG16250.027.468345
CTGTCGC15750.027.426659
GTCTTAC10700.027.2106911
CTCGCTC16950.027.1834373
TCTCGCT16900.026.8378642
GGAGTGC66700.025.5809485
GTATTGG8600.025.3913731
GAGTGCA72850.024.903776
GTGTAAG11550.023.9477651
GTATAAG7400.023.6071151
GTATTAC6550.023.3368031
GTGTTAC10300.023.320621
GTCTCAC32500.022.8444211
GGGTTTC45950.022.6523841
CTGTTGC30050.022.5208829
Produced by FastQC (version 0.11.5)