FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338100
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC21750.034.1327551
GTCTCGC10750.032.4984931
TCTGTCG10150.031.917118
TCGCTCT12850.029.6939814
GTATTAT6400.028.4308931
CTGTCGC13050.026.4793829
GTATTAG7750.026.2958261
GGTATAC5050.025.9424861
TCTCGCT14350.025.0860522
GTCTTAC9200.024.5247341
GTGTTAG16500.024.2610281
GTATAGG5450.024.038451
TAGTACT5450.023.77784
GTTCTAC7050.023.7448351
CTCGCTC14900.023.676353
TTCACCG7750.023.2238375
GTGTAAG10400.023.0946331
GTGTTAA9500.022.9841331
TTGCTCT31750.022.9019954
GTATAAC4204.9112714E-1122.52811
Produced by FastQC (version 0.11.5)