FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449302
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10850.034.2514231
TCTGTCG10700.032.96838
GTCTTGC18050.031.8924621
TCGCTCT12000.029.9991174
CTCGCTC12150.029.037963
TATACCG1000.00628598628.7991495
CTGTCGC15250.027.3805629
CGCTCTG12700.026.6448845
GGTATAC3850.026.4975361
TCTCGCT14450.026.410762
GTGTTAC7250.025.1269741
CGTTTCG1450.001278281724.8248279
GTCTCAC22950.023.8131471
GTCGTGC2303.0237992E-522.1772881
GGTTAAC4006.493792E-1021.8604661
GTACTAA4709.094947E-1221.705431
GTGTAAA10550.021.4115161
GGTCTAT5450.021.3925081
GTATTAA5151.8189894E-1221.2237551
GTCCTAC5500.021.1980291
Produced by FastQC (version 0.11.5)