FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436042
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10850.041.7978558
GTCTCGC10400.039.9495121
TCGCTCT12950.035.5829964
CTGTCGC13550.034.0003859
TCTCGCT12800.032.0809252
CTCGCTC13800.030.2782613
GTCTTGC23950.029.825691
CGCTCTG14950.026.9698895
GTATTAG7600.026.8543341
GGTATAC4850.025.5491891
GTATAAA10950.025.2952791
GTATTAT7250.025.13461
GTGTTAG15400.024.1390081
GTCTTAC9600.023.5375041
TAATACT7400.023.3513434
GTATTGG8450.023.2904031
GTCTAAC5450.022.7364351
GTATAGT6500.022.4277951
GTATTAC5600.022.1274241
GGGTTTC43650.022.0424391
Produced by FastQC (version 0.11.5)