FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436042
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10450.039.9570738
GTCTTGC24050.036.35681
TCGCTCT11200.032.7832454
GTATTAG7200.032.3844831
TATTACG900.003754208332.000222
GTATTAA8100.031.4849131
CTGTCGC13550.030.8156039
GTCTCGC10100.030.3003331
CGCTCTG13100.028.5780015
GTACTAT5400.028.336421
GTGTTAC8400.026.8906841
GTCTTAC8800.025.6683831
GTATTAT7200.025.3003791
TTGCTCT31650.025.2492834
GTCGACC2401.7006696E-623.9972088
CTTGCTC30050.023.7196523
GTATAGG5850.023.6655831
TCTTGCT35850.023.4980682
GGTATAC4353.6379788E-1223.4508321
GTATAGA7850.023.205441
Produced by FastQC (version 0.11.5)