FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412916
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9800.040.40448
GTCTCGC12350.034.776981
GTCTTGC22900.033.3780251
TCGCTCT12650.033.0122074
GTGTTAT7700.031.1982381
CTGTCGC14750.029.2854089
TACGCGG1000.006285375428.7997065
GTGTTAC9300.027.3963011
CGCTCTG14100.027.0635555
TCTCGCT15700.026.1414852
GTCTTAC9750.026.1318571
CTCGCTC15750.025.1441653
GTATTAG8450.024.9832041
GTATTAA8200.024.8571321
GTATAAT6500.024.6386071
GTGTAGC11900.023.8574771
TTGCTCT31350.023.655754
GTACTAT6050.022.8615171
CTTGCTC29650.022.8274923
GTGTAAG11900.022.6340161
Produced by FastQC (version 0.11.5)