FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2695143
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.046.948718
GTCTCGC11450.046.517671
TCGCTCT12750.040.0896844
TCTCGCT14400.037.524392
CTCGCTC14750.035.6570783
CTGTCGC14550.035.6222769
CGCTCTG14100.033.1878175
GTCTTGC23700.032.6330831
GTGTTAG14950.027.3304881
TTGCTCT30100.026.0702044
GTATAGA7850.025.095431
GTCTCAC26350.024.920831
GTGTTAT8100.023.4201091
CTTGCTC29250.023.1535153
GTGTAAC6650.023.0408361
GGCTAGA15200.022.560821
GGATAGT6350.021.8313431
GTATTAC5700.021.760791
GGTATAC4709.094947E-1221.7335551
GTCTAGC8750.021.6803281
Produced by FastQC (version 0.11.5)