FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484572
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10850.041.1360448
CTGTCGC13200.033.2672169
GTCTCGC10500.031.9243931
GTCTTAC9000.029.9580361
GTCTTGC24500.027.6611981
GTGTATA13700.027.1271211
TCGCTCT12550.026.3881914
GTGTTAG17300.025.6943631
GTATTAC5750.025.3464011
GTATTAG8500.024.8618351
GTATTGG8550.024.7164441
CGCTCTG13900.024.343255
GTATTAT6950.023.0670451
TATACTG7950.022.6395825
CCGGTTA1600.00226752622.4962739
TCTCGCT14500.022.3550742
CTCGCTC14300.022.1640033
GTGTAAT10200.022.1470361
GTATAAT7650.021.9088971
GTGTAAC7700.021.7666321
Produced by FastQC (version 0.11.5)