FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2517554
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10950.045.3609778
CTGTCGC13900.037.8055429
GTACTAT4800.031.8877471
GTCTCGC12700.030.9910181
TCGCTCT13000.029.352764
CGCTCTG14950.027.450495
GTGTAAC7450.027.3935031
GTCTTGC22700.026.9711381
GTGTAAT9750.026.911861
CTCGCTC14350.025.603673
GTATTAG7350.024.7912521
TCTCGCT14850.024.7415962
GTGTTAG17450.023.8081261
TTACACT10700.022.8776594
GTGTTAC9600.022.7769621
GTATTCG1600.002109873622.7769621
GTATAAC4505.456968E-1222.6757341
GTATAGT6850.022.3446981
GTACTGG10750.021.696381
GTATAAG6750.021.5959381
Produced by FastQC (version 0.11.5)