FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448210
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10800.043.8670921
TTCGCGC700.001094461141.135858
TCTGTCG11400.039.1512228
CTGTCGC12350.036.721749
TCTCGCT13550.035.0928042
TCGCTCT13700.033.110614
GTATAGG5600.031.2372231
CTCGCTC14650.030.9824963
GTCTTGC24200.029.817351
CGCTCTG14900.028.0277965
GTGTAGA13100.027.2630231
GTATAGC5700.025.5743351
GTGTAAC7800.025.2300661
GTATTAG9550.024.4228211
GTACGAT1500.001449264524.295621
GTTTCAC42750.024.0997963
GTATTAT7600.023.975941
GTGTAAA16350.023.626931
TAATCCG1851.9192309E-423.3521445
TCTCGAT9550.022.6139497
Produced by FastQC (version 0.11.5)