FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448210
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11050.033.2264488
GTCTCGC11950.031.0820671
GTCTTGC24050.030.8882081
TGTACGA1555.7704292E-527.8701882
TCGCTCT13200.026.1794824
CTGTCGC15200.025.5756239
GTACGAG1450.001194933125.1136381
GTATATA9900.025.0121691
TCTCGCT15100.024.794012
TACACCG2651.4746547E-724.450655
CTCGATC10500.023.9968788
GTCTCAC31600.023.969221
TCTCGAT10550.023.8826647
GTATAAC5800.023.8579561
GTGTAAG13800.023.7487661
CTCGCTC14700.023.5090683
GGTATAC5300.023.3604221
TCGATCT10850.023.2227849
CGCTCTG13950.022.7076515
CGTAACC1600.002265703422.499372
Produced by FastQC (version 0.11.5)