FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812985
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12750.038.393088
GTCTCGC12600.035.7938771
TCGCTCT14450.030.3873714
CGCTCTG16050.029.1520715
CTCGCTC14700.028.8922393
TCTCGCT15350.027.7126392
CTGTCGC18650.027.405259
GTATAAC5000.026.6165641
GTGTTAC11000.026.2132821
GTATAGC6300.025.8185351
GTCTTGC26500.025.3889681
GTACTAG4500.024.6449681
GTATAGA7650.024.1617321
GTACCGT1500.001558048723.9956786
GTGTAAT11750.023.9108621
TCTATCG1600.002267790522.4959498
TTACACT13850.022.3485744
GTGTTAG18900.021.5154481
GTATTGT9400.021.2366221
GTTATCG1700.003224243221.1726559
Produced by FastQC (version 0.11.5)