FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027883
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9200.048.519978
CTGTCGC11750.041.05999
GTCTCGC10200.037.8603861
GTCTTGC19300.035.4878651
TCGCTCT10600.033.9585154
GTATAAT5250.033.3089751
CGCTCTG11950.031.9295275
CTCGCTC11700.030.1601833
GTGTTAG14100.029.9721721
GCGCGAT5700.029.055879
TCTCGCT11950.028.9265792
GCGTAGT2155.8678233E-727.1119581
GTATTAC5400.026.9864371
GTATTAT6500.026.9034021
TTGCTCT24900.026.8885964
TGGCGCG7200.025.9990527
GTATTGA6550.025.5856151
TAGTGCG1701.0806638E-425.4145989
TAATACT6950.024.8605634
GTCTTAC8300.024.580421
Produced by FastQC (version 0.11.5)