FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027883
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8950.045.5411451
TCGCTCT9700.043.784194
TCTGTCG9550.040.7020958
GTCTTGC18000.037.0539321
TCTCGCT11550.033.7148062
CGCTCTG12050.032.2568785
CTCGCTC12300.032.1888433
CTGTCGC12700.030.0399029
GTGTTAG13850.027.2888161
GGGTTTC32250.026.196271
TTACACT9900.025.4489524
GTGCTAC7000.025.4084131
GTTTCAC30400.025.1003673
GTTAGCC12050.024.4931473
GTGTAAA11650.024.1725231
GGTATAC4351.8189894E-1223.850811
TAATACT6350.023.8057924
GTATTGG6300.023.5263061
GTCTCAC24100.023.3701151
GTCTTAC8350.023.0755021
Produced by FastQC (version 0.11.5)