FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909374
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11150.040.6802948
GTCTCGC12300.037.3077471
TCGCTCT13500.036.2629934
CTGTCGC14800.030.6492359
TCTCGCT14800.029.6854272
GTCTTGC25950.028.6303331
GTGTTAC10450.028.5779551
GTATTAG7500.028.1643891
CTCGCTC15750.027.8948753
GTATTAT9650.027.1730751
CGCTCTG15550.025.9266385
GTATTAA8150.024.130691
GTACTAG4600.023.7518271
GTGTAAA17050.023.0692231
GTATAGA7050.022.729881
GTATTAC6850.022.3301851
GTCTCAC32100.021.7836061
GTCTTAC11450.021.6290281
TTTACGA2003.246476E-421.6070982
GTTTCAC44500.021.0406193
Produced by FastQC (version 0.11.5)