FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823813
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13800.045.3810238
GTCTTGC28400.039.4041751
TCGCTCT13950.037.6682174
GTCTCGC13550.037.1923181
GCGTAAT1301.4245363E-534.2051541
CTGTCGC18750.033.016529
CTCGCTC15700.032.552563
TCTCGCT16400.031.6749552
CGTTTCG1402.8859453E-530.8501539
CGCTCTG16450.030.1932335
TTGCTCT39900.029.406354
GTATTAG9050.027.8428671
CTTGCTC37000.027.4311373
GTGTTAC12350.026.4039781
GTATTAT9400.026.017751
GTGTAAC9100.025.2466581
GTATAAT8750.023.7155721
TCTTGCT42550.023.2299562
GTATTAC6400.023.1597391
GCTCTGT68200.022.376166
Produced by FastQC (version 0.11.5)