FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2697131
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG750.001535286338.399359
TCTGTCG9400.036.762618
TCGCTCT12000.035.396124
GTCTCGC11950.034.1080551
GTCTTGC27250.032.0517351
CTCGCTC13150.029.576463
GTATAGC6200.029.348451
TCTCGCT13600.029.1274172
CTGTCGC12950.028.3547339
CGCTCTG14200.027.8842685
GTGTATA12650.027.0423341
GTGTTAG19550.026.8054181
GTGTTAC12200.026.2500251
GTATTAG8450.025.8405281
GCGGTAC1400.001039064125.7229143
GTGTAAA15150.023.5407471
GTATAGG6600.023.1585921
GGTATAC5050.023.0603261
GTATAGT7450.022.4702761
TTGCTCT35300.021.8219724
Produced by FastQC (version 0.11.5)