FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776924
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11400.040.418378
GTCTCGC11550.035.3227461
GTATTAG8700.035.1704941
CTGTCGC14700.033.3045129
TCGCTCT12300.031.0206534
GTCTTGC26400.030.9074021
CGCTCTG13250.028.7965325
GTATAGT6150.028.4305021
TCTCGCT14450.027.9098052
GTGTATA14200.026.6786231
GTATTAT7750.026.3211441
CTCGCTC14950.025.5312023
TGTACCG2001.1261998E-525.1969665
GCGTAAA1801.4718968E-424.2843881
GTGTTAT11300.023.2098581
GTGTAGG14200.023.0872691
GGTATAC5850.022.4163571
GTCTCAC32100.022.2417761
GTGTAAT11350.021.8238561
GTCTTAC10300.021.219371
Produced by FastQC (version 0.11.5)