FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776924
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13600.039.1675578
GTATACG2352.7284841E-1134.6664541
GTCTTGC26850.033.099511
CTGTCGC17200.032.6437349
GTCTCGC11800.031.381421
TCGCTCT14250.028.792934
CGCTCTG14450.027.3976235
GTATTAG9000.026.3324991
TACGACT1400.001042599625.7079754
GTCGTAA1450.00108373225.5379831
GTATTAA9050.025.368671
CTCGCTC16150.024.9598163
GTGTAAG13700.024.8669131
GTCTCAC32400.024.4581381
TCTCGCT15250.024.1162052
GTGTTAG20000.023.6992471
GTCTTAC12650.023.4182281
GCGTAAT1901.8997725E-423.3874151
GTACTAG5450.023.1013321
GTGTTAC11400.022.7377641
Produced by FastQC (version 0.11.5)