FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852788
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11750.036.764858
GTCTCGC12350.035.9734341
TCGCTCT13150.034.490234
GTCTTGC25250.033.4592061
CTGTCGC13650.032.7057469
GTCTTAC10950.029.9307381
GTATTAG10300.028.9911631
CTCGCTC16200.028.9016973
TCTCGCT14650.028.5177562
CGCTCTG15200.026.5231915
GTATTCG1701.0004457E-425.7052231
GTGTAAC9550.025.1668931
GTGTTAC12500.024.471371
TTGCTCT37100.024.0618554
TAAACCG2151.9718047E-523.4390985
CTTGCTC35050.023.4283583
GTGTTAG19600.023.0385231
GTCTCAC33000.022.7322441
GTATAGT7800.022.4096831
TTCACCG9050.022.273625
Produced by FastQC (version 0.11.5)