FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852788
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12300.042.7221578
GTCTCGC13200.033.6750371
CGTTTCG1501.2000382E-633.592499
GTCTTGC26200.032.8010251
CTGTCGC18850.029.7864449
TCGCTCT14950.029.3718784
TCTCGCT16250.026.186652
GGTATAC6250.026.0779461
GTCTTAC10800.026.0669731
GTCTCAC35000.026.0356141
GTGTTAC12200.025.50471
GTGTTAT10550.025.2802161
GTGTAAG14950.025.273171
CTCGCTC16350.024.6563343
GTATTAT9350.024.5629671
GTATCGC1851.5819666E-424.0275941
TTGCTCT36750.023.897124
GTGTTAG20400.023.605541
GCGCTAG2201.8870644E-523.5725251
ATACCGG1550.00188588523.2210226
Produced by FastQC (version 0.11.5)