FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2686169
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11150.041.320188
GTCTCGC12450.037.4280551
TCGCTCT13550.036.6557924
GTCTTAC10500.032.40371
CGCTCTG15350.030.952285
GTCTTGC24300.030.4382571
GTGTAAC9550.028.6565861
CTGTCGC14850.028.6010959
GTGTTAC11150.027.8612441
GTATTAC6450.027.5218451
TCTCGCT15500.026.9864962
CTCGCTC16950.025.9055583
CCGTTCG1701.0828874E-425.4073529
ACCGTGT9050.023.863268
GTATAAC5300.023.7246111
GTCCTAC9000.023.0113221
TTACACT14700.022.0357594
GAGTGCA63600.021.958456
GTGTAAT10800.021.9155461
GTCTCAC32450.021.8817771
Produced by FastQC (version 0.11.5)