FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420737
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11400.048.6313328
GTCTCGC10550.043.6829221
TCGCTCT12100.039.272534
GTCTTGC21050.036.260841
CTGTCGC16200.035.5553789
CTCGCTC14300.030.7131333
GTATTAA8100.030.2258171
TCTCGCT13650.030.0695112
CGCTCTG14150.030.021055
GTATTAG7500.029.763541
TTCACCG7100.028.3942245
GTATTAC5400.028.003331
GGGTTTC41450.026.579761
TTGCTCT32400.025.999874
GTATTAT7950.025.3615061
GTTTCAC38750.025.0837463
TAATACT6800.024.352824
TCTGTCA31700.024.0755888
GTGTTAC8750.023.8656941
GTATAAT8950.023.3323821
Produced by FastQC (version 0.11.5)