FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282575
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10650.045.9714478
TCGCTCT12650.039.27244
GTCTCGC12150.038.5367661
CTGTCGC13400.038.1489339
GTCTTGC22350.035.130731
GTATTAG6950.032.130321
GTATTAC5700.030.3301531
CTCGCTC14150.030.0281873
TCTCGCT15150.029.4793032
CGCTCTG13700.027.3282385
GTCTTAC9650.026.872831
GTGTAAC7300.026.6427541
GTATTAA7400.026.2827171
TTGCTCT29600.025.0538444
GGTATAC3751.2732926E-1124.9718271
GTGTAAA13600.024.8941421
GTGTTAG15500.024.6310181
GTATAAT7750.024.166281
GTTTCAC36700.023.9401723
GGGTTTC41450.023.461051
Produced by FastQC (version 0.11.5)