FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282575
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10900.040.3031581
TCTGTCG10700.039.7007988
TCGCTCT11650.037.6994444
GTCTTGC22400.034.7225381
GTATTCG1708.3040504E-833.8904041
GTATTAC4800.031.5074861
CTGTCGC13950.030.4515119
CTTATCG1204.2277068E-429.99999
TCTCGCT13950.028.9100952
CTCGCTC14100.028.595653
CGCTCTG13850.027.5522545
TTGCTCT31750.025.1715724
GTACTAT5500.024.8786371
GTGTTAT9000.024.8058951
GTACTAG4650.024.780081
GGGTTTC39250.024.5867351
GTATATA9150.024.3992421
GTCCTAC6850.024.180931
CTGTTGC24850.024.048219
GTCTCAC28250.023.453361
Produced by FastQC (version 0.11.5)