FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955508
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.040.1714328
GTCTCGC13150.039.4338261
TCGCTCT15350.034.709894
TCTCGCT16650.032.442122
CTGTCGC15800.031.8985449
GTATTAA7250.031.7888031
GTCTTGC27550.030.8476831
CTCGCTC17300.030.8016723
ATAGTAC4950.030.5494063
CGCTCTG16650.028.5403695
TACACCG2654.9167284E-927.169655
GTCTCAC34750.026.736041
GTATTAG8700.025.6628321
TTGCTCT38700.025.3021744
TTATACT6850.024.1750374
CTTGCTC36650.023.3809243
GTGTAAC8650.023.3133181
GTATTGG9950.023.1626931
GTATTAT6550.023.0908641
GTGTAAA15050.022.9703121
Produced by FastQC (version 0.11.5)