FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955508
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.041.32168
GTCTCGC12600.041.152471
TCGCTCT13900.034.1869354
CTGTCGC16200.033.3332259
GTCTTGC25800.032.658791
TCTCGCT17650.028.561912
GTACGAA1907.5434127E-626.5325131
GTCTCAC33350.025.9130971
CTCGCTC17400.025.6550883
GTCTTAC11750.025.1292721
GTTTCAC40250.024.8645153
TTGCTCT37900.024.3165444
CGCTCTG16700.024.1436335
GTATTAA7800.024.0056041
GGGTTTC47700.021.5899491
GTATTAC7100.021.3007491
GTCGTAA1700.003216555821.1814161
GTATAAT8950.020.9210851
CTTGCTC36550.020.4869353
TCACTCT39500.020.2328454
Produced by FastQC (version 0.11.5)