FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489181
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9200.041.4779438
GTCTCGC11800.036.621661
TCGCTCT11100.035.67544
CTGTCGC12300.032.1948749
GTCTTGC23250.029.7383631
TCTCGCT13700.029.4398942
CTCGCTC13900.027.457993
GTATTAT7300.026.6384941
TGTATCG1450.00127751724.8273965
GTACGGT1751.3153524E-424.689993
GCGTTAT1801.5908903E-424.007531
CGCTCTG14350.023.58175
GCGTAGT1851.9156068E-423.3586791
GTCTCAC28100.022.8114281
ATAGTAC4800.022.5038973
GTACTAC4609.094947E-1221.919921
GCGCTAG2303.3089447E-521.919921
GTGTTAT8250.021.825031
GTCTAAC4951.8189894E-1221.8250271
TAACACG4659.094947E-1221.6772544
Produced by FastQC (version 0.11.5)