FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489181
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11700.041.2354971
TCTGTCG10900.040.2933438
TCGCTCT12900.035.1625864
CTGTCGC14650.033.9110689
GTCTTGC22500.031.3635961
GTCTTAC8500.031.344771
CTCGCTC14450.030.8925533
TCTCGCT15500.030.1970122
CGCTCTG14800.029.6754995
GCGTTAT1450.001276606524.8304331
TTACTCT13500.024.5331884
GTGTTAA9150.024.396241
GTATAGC5050.024.2404041
TTGCTCT33450.023.8922374
GTATTGG8150.023.8554951
GTATTAG8250.023.5663381
GTCTCAC27650.023.1780661
GGGTTTC40700.022.8232561
GGATACG1902.2979314E-422.7394491
GTTTCAC36200.022.67393
Produced by FastQC (version 0.11.5)