FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484865
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10000.046.799158
GTCTCGC10800.039.3516241
CTGTCGC12900.037.95289
TCGCTCT12050.036.447474
GTCTTGC22800.035.0689281
GTCTTAC9250.031.9283541
TCTCGCT12350.031.4964182
CGCTCTG12650.030.1654595
CTCGCTC13450.028.3797973
GTACTAC3950.027.3544831
GTATTAA6300.026.2979351
GTTTCAC36850.025.407483
GGGTTTC41400.024.8811281
TCCGTAC1450.001275348824.8346313
GTGTAAC6800.024.3642641
TTACTCT12800.024.1870614
TCTTGCT34350.023.2771932
GTCTCAC26350.022.9632341
GGCTAGA14750.022.953041
TACACTG14800.022.864455
Produced by FastQC (version 0.11.5)