FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586032
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13050.046.9025761
GTACTAT4550.041.1481441
TCTGTCG10500.039.7711568
GTCTTGC22200.038.5995521
TCGCTCT15350.037.055124
TCTCGCT15900.035.3252942
CTCGCTC17050.032.938193
TATACCG950.004891618630.3155845
CGCTCTG16800.029.1426565
GTGTTAG17100.028.2141511
CTGTCGC15600.026.3075129
GTATTAA4950.026.1851831
CTATCGC1658.821864E-526.1851831
TTGCTCT30650.026.0748624
GGTATAC4450.025.8909681
GTATTAC5350.025.573381
GTATTAT7350.025.4726621
GGGTTTC40550.025.3941381
GTTTCAC37700.025.2093773
GTTAGCC13050.024.2756943
Produced by FastQC (version 0.11.5)